Issue 17, 2014

Up-regulating pyocyanin production by amino acid addition for early electrochemical identification of Pseudomonas aeruginosa

Abstract

This work focuses on developing a faster method for electrochemically detecting a Pseudomonas aeruginosa infection through the addition of amino acids to cell culture samples. We performed square-wave voltammetry measurements of pyocyanin produced by P. aeruginosa using commercially available carbon-based electrodes connected to a Ag/AgCl reference. The electrochemical response resulting from the production of pyocyanin by bacteria was measured in the presence of various amino acids while varying three different culturing parameters: liquid media type (trypticase soy broth vs. M63 minimal media); concentration of amino acids in the solution; and initial concentration of the P. aeruginosa in the solution. Our results demonstrate a faster and stronger electrochemical response in media containing tyrosine and valine at elevated concentrations, lending promise to using amino acids as up-regulatory molecules for faster bacterial detection.

Graphical abstract: Up-regulating pyocyanin production by amino acid addition for early electrochemical identification of Pseudomonas aeruginosa

Supplementary files

Article information

Article type
Paper
Submitted
28 Apr 2014
Accepted
23 Jun 2014
First published
23 Jun 2014

Analyst, 2014,139, 4241-4246

Author version available

Up-regulating pyocyanin production by amino acid addition for early electrochemical identification of Pseudomonas aeruginosa

H. J. Sismaet, T. A. Webster and E. D. Goluch, Analyst, 2014, 139, 4241 DOI: 10.1039/C4AN00756E

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