Issue 11, 2014

Subnanomolar detection of ochratoxin A using aptamer-attached silver nanoparticles and surface-enhanced Raman scattering

Abstract

We report a new approach for the detection of ochratoxin A (OTA) by introducing surface-enhanced Raman scattering (SERS). This method is based on different adsorption propensities of Raman reporter Cy5-tagged OTA aptamer. The OTA aptamer could be easily adsorbed onto the surface of silver nanoparticles (AgNPs) to give fairly strong SERS signals, whereas such bindings would be hampered in the presence of OTA. On the addition of OTA in the concentration range of 0.1–10 nM, the SERS signals appeared to decrease by ∼40%, since OTA's aptamer could not adsorb onto the surface of AgNPs due to binding with OTA. We verified our results against a control experiment with warfarin, which did not affect the SERS signals. A nanomolar detection limit of OTA was achieved using the current SERS-based method.

Graphical abstract: Subnanomolar detection of ochratoxin A using aptamer-attached silver nanoparticles and surface-enhanced Raman scattering

Article information

Article type
Communication
Submitted
23 Feb 2014
Accepted
28 Mar 2014
First published
28 Mar 2014

Anal. Methods, 2014,6, 3573-3577

Subnanomolar detection of ochratoxin A using aptamer-attached silver nanoparticles and surface-enhanced Raman scattering

E. Ganbold, C. M. Lee, E. Cho, S. J. Son, S. Kim, S. Joo and S. I. Yang, Anal. Methods, 2014, 6, 3573 DOI: 10.1039/C4AY00440J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements