Issue 44, 2014

One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1

Abstract

An efficient one-pot chemical synthesis of snake venom toxin Mambalgin-1 was achieved using an azide-switch strategy combined with hydrazide-based native chemical ligation. Synthetic Mambalgin-1 exhibited a well-defined structure after sequential folding in vitro. NMR spectroscopy revealed a three-finger toxin family structure, and the synthetic toxin inhibited human acid-sensing ion channel 1a.

Graphical abstract: One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1

Supplementary files

Article information

Article type
Communication
Submitted
29 Jan 2014
Accepted
24 Feb 2014
First published
25 Feb 2014

Chem. Commun., 2014,50, 5837-5839

Author version available

One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1

M. Pan, Y. He, M. Wen, F. Wu, D. Sun, S. Li, L. Zhang, Y. Li and C. Tian, Chem. Commun., 2014, 50, 5837 DOI: 10.1039/C4CC00779D

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