Issue 58, 2014

Distance-determined sensitivity in attenuated total reflection-surface enhanced infrared absorption spectroscopy: aptamer–antigen compared to antibody–antigen

Abstract

Distance-dependent signal intensity in immunoassay by attenuated total reflection-surface enhanced infrared absorption spectroscopy is demonstrated by controlling the distance of target proteins away from the enhancement substrate. Based on this optical near-field effect, sensitive detection of protein molecules with a detection limit of 0.6 nM and investigation of the kinetics and thermodynamics of protein–aptamer/antibody interactions can be achieved.

Graphical abstract: Distance-determined sensitivity in attenuated total reflection-surface enhanced infrared absorption spectroscopy: aptamer–antigen compared to antibody–antigen

Supplementary files

Article information

Article type
Communication
Submitted
14 Mar 2014
Accepted
27 May 2014
First published
27 May 2014

Chem. Commun., 2014,50, 7787-7789

Distance-determined sensitivity in attenuated total reflection-surface enhanced infrared absorption spectroscopy: aptamer–antigen compared to antibody–antigen

W. Bao, Z. Yan, M. Wang, Y. Zhao, J. Li, K. Wang, X. Xia and Z. Wang, Chem. Commun., 2014, 50, 7787 DOI: 10.1039/C4CC01920B

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