Issue 66, 2014

A modular tyrosine kinase deoxyribozyme with discrete aptamer and catalyst domains

Abstract

We assess the utility of integrating a predetermined aptamer DNA module adjacent to a random catalytic DNA region for identifying new deoxyribozymes by in vitro selection. By placing a known ATP aptamer next to an N40 random region, an explicitly modular DNA catalyst for tyrosine side chain phosphorylation is identified. The results have implications for broader identification of deoxyribozymes that function with small-molecule substrates.

Graphical abstract: A modular tyrosine kinase deoxyribozyme with discrete aptamer and catalyst domains

Supplementary files

Article information

Article type
Communication
Submitted
03 Jun 2014
Accepted
25 Jun 2014
First published
30 Jun 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2014,50, 9317-9320

Author version available

A modular tyrosine kinase deoxyribozyme with discrete aptamer and catalyst domains

V. Dokukin and S. K. Silverman, Chem. Commun., 2014, 50, 9317 DOI: 10.1039/C4CC04253K

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements