Issue 74, 2014

Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels–Alder reaction

Abstract

Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels–Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.

Graphical abstract: Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels–Alder reaction

Supplementary files

Article information

Article type
Communication
Submitted
06 Jun 2014
Accepted
11 Jul 2014
First published
22 Jul 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2014,50, 10827-10829

Author version available

Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels–Alder reaction

H. Bußkamp, E. Batroff, A. Niederwieser, O. S. Abdel-Rahman, R. F. Winter, V. Wittmann and A. Marx, Chem. Commun., 2014, 50, 10827 DOI: 10.1039/C4CC04332D

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