Issue 86, 2014

eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA–gold affinity interactions

Abstract

We report a simple electrochemical method referred to as “eMethylsorb” for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.

Graphical abstract: eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA–gold affinity interactions

Supplementary files

Article information

Article type
Communication
Submitted
27 Aug 2014
Accepted
09 Sep 2014
First published
09 Sep 2014

Chem. Commun., 2014,50, 13153-13156

Author version available

eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA–gold affinity interactions

A. A. I. Sina, S. Howell, L. G. Carrascosa, S. Rauf, M. J. A. Shiddiky and M. Trau, Chem. Commun., 2014, 50, 13153 DOI: 10.1039/C4CC06732K

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