Issue 16, 2015
From the journal:

Chemical Communications

Highly sensitive detection of CpG methylation in genomic DNA by AuNP-based colorimetric assay with ligase chain reaction†

Fengxia Su,a   Limei Wang,a   Yueying Sun,a   Chenghui Liu,b   Xinrui Duanb  and  Zhengping Li*ab  

* Corresponding authors

a Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P. R. China
E-mail: lzpbd@snnu.edu.cn
Fax: +86 29 81530859

b Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, Shaanxi Province, P. R. China

Abstract

We have developed a new ligase chain reaction-based colorimetric assay for detection of DNA methylation with ultrahigh sensitivity and selectivity. Using the proposed assay, as low as 0.01 fM methylated DNA can be detected by visualization of color changes of gold nanoparticles with the naked eye.

Graphical abstract: Highly sensitive detection of CpG methylation in genomic DNA by AuNP-based colorimetric assay with ligase chain reaction

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Article information

DOI
https://doi.org/10.1039/C4CC07688E
Article type
Communication
Submitted
29 Sep 2014
Accepted
19 Jan 2015
First published
19 Jan 2015

Chem. Commun., 2015,51, 3371-3374

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Highly sensitive detection of CpG methylation in genomic DNA by AuNP-based colorimetric assay with ligase chain reaction

F. Su, L. Wang, Y. Sun, C. Liu, X. Duan and Z. Li, Chem. Commun., 2015, 51, 3371 DOI: 10.1039/C4CC07688E

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