Issue 26, 2015
From the journal:

Chemical Communications

Light-cleavable rapamycin dimer as an optical trigger for protein dimerization†

Kalyn A. Brown,ab   Yan Zou,b   David Shirvanyants,c   Jie Zhang,b   Subhas Samanta,a   Pavan K. Mantravadi,a   Nikolay V. Dokholyanc  and  Alexander Deiters ORCID logo *ab  

* Corresponding authors

a Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
E-mail: deiters@pitt.edu

b Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204, USA

c Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA

Abstract

Rapamycin-induced protein heterodimerization of FKBP12 and FRB is one of the most commonly employed switches to conditionally control biological processes. We developed an optically activated rapamycin dimer that does not induce FKBP12-FRB dimerization until exposed to light, and applied it to control kinase, protease, and recombinase function.

Graphical abstract: Light-cleavable rapamycin dimer as an optical trigger for protein dimerization

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Article information

DOI
https://doi.org/10.1039/C4CC09442E
Article type
Communication
Submitted
26 Nov 2014
Accepted
17 Feb 2015
First published
26 Feb 2015

Chem. Commun., 2015,51, 5702-5705

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Light-cleavable rapamycin dimer as an optical trigger for protein dimerization

K. A. Brown, Y. Zou, D. Shirvanyants, J. Zhang, S. Samanta, P. K. Mantravadi, N. V. Dokholyan and A. Deiters, Chem. Commun., 2015, 51, 5702 DOI: 10.1039/C4CC09442E

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