Issue 15, 2015

Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells

Abstract

We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.

Graphical abstract: Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells

Supplementary files

Article information

Article type
Communication
Submitted
06 Dec 2014
Accepted
07 Jan 2015
First published
23 Jan 2015

Chem. Commun., 2015,51, 3266-3269

Author version available

Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells

S. V. Nair, M. A. Witek, J. M. Jackson, M. A. M. Lindell, S. A. Hunsucker, T. Sapp, C. E. Perry, M. L. Hupert, V. Bae-Jump, P. A. Gehrig, W. Z. Wysham, P. M. Armistead, P. Voorhees and S. A. Soper, Chem. Commun., 2015, 51, 3266 DOI: 10.1039/C4CC09765C

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