Enzyme-driven i-motif DNA folding for logic operations and fluorescent biosensing

Abstract

DNA nanodevices capable of “NOR” and “NAND” logic operations were developed using enzymatic reactions to generate an acidic pH gradient and drive the conformation change of cytosine-rich DNA. Due to the high selectivity and sensitivity of the enzymatic reactions in driving DNA logic gates, novel fluorescent biosensors were further designed for enzyme activity assay and glucose sensing.