Issue 23, 2015
From the journal:

Chemical Communications

Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging†

Amnon Bar-Shir,ab   Yajie Liang,ab   Kannie W. Y. Chan,ab   Assaf A. Giladabc  and  Jeff W. M. Bulte*abcdef  

* Corresponding authors

a Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

b Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

c F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, Maryland, USA

d Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

e Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

f Department of Chemical & Biomolecular Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
E-mail: jwmbulte@mri.jhu.edu

Abstract

Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

Graphical abstract: Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

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Article information

DOI
https://doi.org/10.1039/C4CC10195B
Article type
Communication
Submitted
21 Dec 2014
Accepted
13 Feb 2015
First published
13 Feb 2015

Chem. Commun., 2015,51, 4869-4871

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Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

A. Bar-Shir, Y. Liang, K. W. Y. Chan, A. A. Gilad and J. W. M. Bulte, Chem. Commun., 2015, 51, 4869 DOI: 10.1039/C4CC10195B

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