Volume 177, 2015

Phase modulation nanoscopy: a simple approach to enhanced optical resolution

Abstract

A new modular super-resolution technique called Phase Modulation Nanoscopy (PhMoNa) has been developed in order to break the optical diffraction barrier in Confocal Laser Scanning Microscopy (LSCM). This technique is based on using spatially modulated illumination intensity, whilst harnessing the fluorophore's non-linear emission response. It allows experimental resolution in both lateral and axial domains to be improved by at least a factor of 2. The work is in its initial phase, but by using a custom built Electro Optical Modulator (EOM) in conjunction with functionalised Ln(III) complexes as probes, a sub-diffraction resolution of ∼60 nm was achieved of selected cellular organelles in long term live cell imaging experiments.

Associated articles

Article information

Article type
Paper
Submitted
15 Aug 2014
Accepted
05 Nov 2014
First published
22 Jan 2015

Faraday Discuss., 2015,177, 507-515

Author version available

Phase modulation nanoscopy: a simple approach to enhanced optical resolution

R. Pal, Faraday Discuss., 2015, 177, 507 DOI: 10.1039/C4FD00158C

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