Issue 5, 2015

A system for ω-transaminase mediated (R)-amination using l-alanine as an amine donor

Abstract

Chiral amines are important building blocks for fine chemicals and pharmaceuticals. Consequently, various biocatalytic routes in particular using ω-transaminases (ω-TAs) have been developed recently. Although catalysts for the synthesis of both enantiomers are available, the application of alanine dependent (R)-selective ω-TAs is less favourable due to the requirement of the more expensive D-alanine as an amine donor. Here we describe an efficient method for (R)-amination using ω-TAs in combination with an alanine racemase (AlaR). In this case, the readily available L-alanine can be used as an amine donor leading to improved atom efficiency and significantly reduced costs.

Graphical abstract: A system for ω-transaminase mediated (R)-amination using l-alanine as an amine donor

Supplementary files

Article information

Article type
Paper
Submitted
01 Dec 2014
Accepted
10 Mar 2015
First published
19 Mar 2015
This article is Open Access
Creative Commons BY license

Green Chem., 2015,17, 2952-2958

Author version available

A system for ω-transaminase mediated (R)-amination using L-alanine as an amine donor

N. Richter, J. E. Farnberger, D. Pressnitz, H. Lechner, F. Zepeck and W. Kroutil, Green Chem., 2015, 17, 2952 DOI: 10.1039/C4GC02363C

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