Issue 4, 2015

Cytotoxic potential of dispirooxindolo/acenaphthoquino andrographolide derivatives against MCF-7 cell line

Abstract

Dispiro andrographolide derivatives have been prepared from isatin/acenaphthoquinone, N-benzyl glycine and andrographolide via azomethine ylide cycloaddition reaction. The cytotoxic effect of the synthesized molecules has been studied against MCF-7 breast cancer cell line. The compounds induced apoptotic cell death as revealed by increased labeling with Annexin-V, decreased polarization of cell mitochondria, and increased reactive oxygen species production. Using FACS and western blot analysis, the compounds were observed to block the cell cycle at S phase. Activation of caspases 7 and 9 suggested that caspase pathways were involved in inducing apoptosis.

Graphical abstract: Cytotoxic potential of dispirooxindolo/acenaphthoquino andrographolide derivatives against MCF-7 cell line

Supplementary files

Article information

Article type
Concise Article
Submitted
15 Oct 2014
Accepted
07 Jan 2015
First published
08 Jan 2015

Med. Chem. Commun., 2015,6, 702-707

Author version available

Cytotoxic potential of dispirooxindolo/acenaphthoquino andrographolide derivatives against MCF-7 cell line

D. Chakraborty, A. Maity, C. K. Jain, A. Hazra, Y. P. Bharitkar, T. Jha, H. K. Majumder, S. Roychoudhury and N. B. Mondal, Med. Chem. Commun., 2015, 6, 702 DOI: 10.1039/C4MD00469H

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