A novel sandwich-type electrochemical aptasensor for sensitive detection of kanamycin based on GR–PANI and PAMAM–Au nanocomposites

Abstract

In this work, we report a novel sandwich-type electrochemical aptasensor for sensitive and selective detection of antibiotic kanamycin. This aptasensor was constructed by assembling graphene–polyaniline (GR–PANI) and polyamidoamine dendrimer–Au nanoparticle (PAMAM–Au) nanocomposites on the surfaces of a glass carbon electrode, which as electrode materials exhibited excellent charge-transport property and loading capacity of biomolecules. Followed by the sandwich-type specific identification, kanamycin detection was realized by outputting a redox current from electro-reduction reaction of H₂O₂ catalyzed by HRP. It was demonstrated that the developed strategy had a wide linear response range from 5 × 10⁻⁶ to 4 × 10⁻² μg mL⁻¹ and a low detection limit of 4.6 × 10⁻⁶ μg mL⁻¹. Additionally, this aptasensor had the advantages in its low cost, high sensitivity, and superb specificity. Thus, it might provide a useful tool for kanamycin determination and related food safety analysis and clinical diagnosis.