Issue 18, 2014

Thermo-responsive gold/poly(vinyl alcohol)-b-poly(N-vinylcaprolactam) core–corona nanoparticles as a drug delivery system

Abstract

Core–corona gold/poly(vinyl alcohol)-b-poly(N-vinylcaprolactam) nanoparticles (gold@PVOH-b-PNVCL NPs) were fabricated via an in situ method, where a gold salt was reduced within the macromolecular aqueous solution. Arrangement of macromolecular chains on the surface of gold cores was studied by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy and infrared spectroscopy. The responsiveness to temperature and the preserved colloidal stability of the gold@PVOH-b-PNVCL NPs above the lower critical solution temperature (LCST) were confirmed by dynamic light scattering and turbidity measurements. The drug loading capacity (DLC of ca. 1.3–2.8 wt%) of the gold@PVOH-b-PNVCL NPs as a drug delivery system (DDS) was tested with Nadolol®, a hydrophilic drug, and the release behaviours were studied at several temperatures. PVOH-b-PNVCL copolymers with an LCST of a few degrees above the biological temperature (37 °C), for example, PVOH180-b-PNVCL110 (LCST of 41 °C), are preferential, due to the slower release at 37 °C, but a faster release at temperatures that are a few degrees higher. The cytocompatibility of the gold@PVOH-b-PNVCL NPs against mouse fibroblastic L929 cells was evaluated via the MTS assay. Cellular uptake within MEL-5 human melanoma cells was studied by confocal laser scanning microscopy, fluorescence-activated cell sorting and TEM techniques and it showed that gold@PVOH-b-PNVCL NPs preferably accumulated within the cellular cytoplasm, with an incubation concentration and period-dependent uptake process. All these results corroborated a general utility of these thermo-responsive gold@PVOH-b-PNVCL NPs for drug delivery and controlled drug release.

Graphical abstract: Thermo-responsive gold/poly(vinyl alcohol)-b-poly(N-vinylcaprolactam) core–corona nanoparticles as a drug delivery system

Supplementary files

Article information

Article type
Paper
Submitted
11 Mar 2014
Accepted
08 May 2014
First published
09 May 2014

Polym. Chem., 2014,5, 5289-5299

Author version available

Thermo-responsive gold/poly(vinyl alcohol)-b-poly(N-vinylcaprolactam) core–corona nanoparticles as a drug delivery system

J. Liu, C. Detrembleur, M. Hurtgen, A. Debuigne, M. De Pauw-Gillet, S. Mornet, E. Duguet and C. Jérôme, Polym. Chem., 2014, 5, 5289 DOI: 10.1039/C4PY00352G

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