Issue 52, 2014
From the journal:

RSC Advances

Enhanced turnover rate and enantioselectivity in the asymmetric epoxidation of styrene by new T213G mutants of CYP 119†

Chun Zhang,‡a   Jing Li,‡a   Bo Yang,b   Fang He,a   Sheng-Yong Yang,*b   Xiao-Qi Yu*c  and  Qin Wang*a  

* Corresponding authors

a Department of Medicinal Chemistry, Luzhou Medical College, Luzhou 646000, China
E-mail: wq_ring@hotmail.com

b State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China
E-mail: yangsy@scu.edu.cn

c College of Chemistry, Sichuan University, Chengdu 610064, China
E-mail: xqyu@scu.edu.cn

Abstract

New CYP 119 T213G mutants were constructed and characterized. Introduction of the T213G mutation into the wild-type CYP 119 significantly enhances the turnover rate for the peroxide-dependent styrene epoxidation 4.4-fold to 346.2 min−1, and the double T213G/T214V mutant improves the ratio of the S- and R-enantiomers of the epoxide products 2- fold to 5.8. The molecular modelling and docking results support our initial design and experimental data.

Graphical abstract: Enhanced turnover rate and enantioselectivity in the asymmetric epoxidation of styrene by new T213G mutants of CYP 119

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Article information

DOI
https://doi.org/10.1039/C4RA04626A
Article type
Paper
Submitted
18 Apr 2014
Accepted
04 Jun 2014
First published
09 Jun 2014

RSC Adv., 2014,4, 27526-27531

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Enhanced turnover rate and enantioselectivity in the asymmetric epoxidation of styrene by new T213G mutants of CYP 119

C. Zhang, J. Li, B. Yang, F. He, S. Yang, X. Yu and Q. Wang, RSC Adv., 2014, 4, 27526 DOI: 10.1039/C4RA04626A

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