Issue 106, 2014

An electrogenerated chemiluminescent biosensor based on a g-C3N4–hemin nanocomposite and hollow gold nanoparticles for the detection of lactate

Abstract

In this article, a new electrochemiluminescent (ECL) biosensor based on a g-C3N4–hemin nanocomposite and hollow gold nanoparticles (HGNPs) was constructed to detect lactate. Firstly, the g-C3N4 nanosheets were prepared through ultrasonication-assisted liquid exfoliation of bulk g-C3N4, which was obtained through polymerizing melamine under 600 °C. Then, the nanocomposites of g-C3N4 nanosheets and hemin were prepared to modify a glassy carbon electrode. Subsequently, HGNPs were self-assembled onto the electrode for adsorbing lactate oxidase to achieve a lactate biosensor. Due to the excellent catalytic effect of g-C3N4–hemin and HGNPs on the luminol/H2O2 ECL system, the as-prepared biosensor exhibited a good response performance to lactate with a linear range of 1.7 × 10−8 to 5.0 × 10−4 M and a detection limit of 5.5 × 10−9 M. In addition, the prepared ECL biosensor exhibited satisfying reproducibility and stability. The g-C3N4–hemin nanocomposite might have great potential application in a luminol/H2O2 ECL system.

Graphical abstract: An electrogenerated chemiluminescent biosensor based on a g-C3N4–hemin nanocomposite and hollow gold nanoparticles for the detection of lactate

Supplementary files

Article information

Article type
Paper
Submitted
04 Sep 2014
Accepted
11 Nov 2014
First published
11 Nov 2014

RSC Adv., 2014,4, 61759-61766

An electrogenerated chemiluminescent biosensor based on a g-C3N4–hemin nanocomposite and hollow gold nanoparticles for the detection of lactate

H. Chen, X. Tan, J. Zhang, Q. Lu, X. Ou, Y. Ruo and S. Chen, RSC Adv., 2014, 4, 61759 DOI: 10.1039/C4RA09616A

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