Hollow AuPt alloy nanoparticles as an enhanced immunosensing platform for detection of multiple analytes

Abstract

Novel hollow AuPt alloy nanoparticles (hAuPt NPs) with a rough surface are prepared by a simple one-step galvanic displacement reaction between Co nanoparticles and a mixture of HAuCl₄ and K₂PtCl₄ under mild conditions. The synthesized hAuPt NPs are decorated with graphene oxide reduced by thionine or thiol functionalized ferrocene, which are utilized as electrochemical immunosensing probes for simultaneous detection of multiple analytes in a signal run. Using carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) as model analytes, this proposed immunosensing shows wide linear range and low detection limits to 0.011 and 0.015 ng mL⁻¹ for CEA and AFP, respectively. The proposed immunosensor for the detection of serum samples is consistent with ELISA. These results suggest that the hAuPt NPs can be used as an immunosensing platform for detection of multiple analytes.