Issue 96, 2014

Construction of a fluorescence turn-on probe for highly discriminating detection of cysteine

Abstract

It is still a challenge to construct probes for discriminating thiols due to the similar structure and reactivities of thiol-containing molecules. We have here developed a Cys specific probe by utilizing the remarkable difference in reactivity toward Cys, Hcy and GSH. The reaction between the designed probe and Cys produces an amino-substituted BODIPY, giving a yellow fluorescence turn-on response. The response to Hcy or GSH shows a red fluorescence turn-on signal, due to the formation of sulfenyl-substituted BODIPY. These distinct fluorescence turn-on responses allow Cys to be distinguished from Hcy and GSH. This probe was also utilized for detection of Cys in living cells and monitoring cystathionine γ-lyase activity in vitro.

Graphical abstract: Construction of a fluorescence turn-on probe for highly discriminating detection of cysteine

Supplementary files

Article information

Article type
Paper
Submitted
25 Sep 2014
Accepted
10 Oct 2014
First published
10 Oct 2014

RSC Adv., 2014,4, 53437-53441

Author version available

Construction of a fluorescence turn-on probe for highly discriminating detection of cysteine

F. Wang, J. An, L. Zhang and C. Zhao, RSC Adv., 2014, 4, 53437 DOI: 10.1039/C4RA11167B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements