Issue 3, 2015

Functionalized graphene-based biomimetic microsensor interfacing with living cells to sensitively monitor nitric oxide release

Abstract

It is a great challenge to develop electrochemical sensors with superior sensitivity that concurrently possess high biocompatibility for monitoring at the single cell level. Herein we report a novel and reusable biomimetic micro-electrochemical sensor array with nitric oxide (NO) sensing-interface based on metalloporphyrin and 3-aminophenylboronic acid (APBA) co-functionalized reduced graphene oxide (rGO). The assembling of high specificity catalytic but semi-conductive metalloporphyrin with high electric conductive rGO confers the sensor with sub-nanomolar sensitivity. Further coupling with the small cell-adhesive molecule APBA obviously enhances the cytocompatibility of the microsensor without diminishing the sensitivity, while the reversible reactivity between APBA and cell membrane carbohydrates allows practical reusability. The microsensor was successfully used to sensitively monitor, in real-time, the release of NO molecules from human endothelial cells being cultured directly on the sensor. This demonstrates its potential application in the detection of NO with very low bioactive concentrations for the better understanding of its physiological function and for medical tracking of patient states.

Graphical abstract: Functionalized graphene-based biomimetic microsensor interfacing with living cells to sensitively monitor nitric oxide release

Supplementary files

Article information

Article type
Edge Article
Submitted
11 Oct 2014
Accepted
21 Jan 2015
First published
28 Jan 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2015,6, 1853-1858

Author version available

Functionalized graphene-based biomimetic microsensor interfacing with living cells to sensitively monitor nitric oxide release

Y. Liu, X. Wang, J. Xu, C. Xiao, Y. Liu, X. Zhang, J. Liu and W. Huang, Chem. Sci., 2015, 6, 1853 DOI: 10.1039/C4SC03123G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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