Issue 2, 2015

Enzyme-mediated single-nucleotide variation detection at room temperature with high discrimination factor

Abstract

We demonstrate a new powerful tool to detect single-nucleotide variation in DNA at room temperature with high selectivity, based on predetermined specific interactions between Lambda exonuclease and a chemically modified DNA substrate structure which comprises two purposefully introduced mismatches and a covalently attached fluorophore. The fluorophore not only acts as a signal reporter in the detection system, but also plays a notable role in the specific molecular recognition between the enzyme and the probe/target hybrid substrate. The method is single-step, rapid, and can be easily adapted to different high-throughput micro-devices without the need for temperature control.

Graphical abstract: Enzyme-mediated single-nucleotide variation detection at room temperature with high discrimination factor

Supplementary files

Article information

Article type
Edge Article
Submitted
04 Nov 2014
Accepted
06 Nov 2014
First published
07 Nov 2014
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2015,6, 1206-1211

Author version available

Enzyme-mediated single-nucleotide variation detection at room temperature with high discrimination factor

T. Wu, X. Xiao, Z. Zhang and M. Zhao, Chem. Sci., 2015, 6, 1206 DOI: 10.1039/C4SC03375B

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