Issue 22, 2014

A fluorescent light-up probe with “AIE + ESIPT” characteristics for specific detection of lysosomal esterase

Abstract

We report a fluorescent light-up probe AIE-Lyso-1 for in situ visualization of lysosomal esterase activity. The probe is based on a salicyladazine fluorogen, which is conjugated with esterase reactive acetoxyl groups and lysosome-targeting morpholine moieties. The probe has characteristics of both aggregation-induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT), which shows significant advantages, such as lysosome-specific targeting, no self-quenching at high concentration, excellent light-up ratio, large Stokes shift, low cytotoxicity, and high specificity to esterase. It has also been used for in situ monitoring of lysosomal esterase activity and tracking lysosomal movements in living cells, which has great potential for the diagnosis of Wolman disease caused by deficiency of lysosomal esterase.

Graphical abstract: A fluorescent light-up probe with “AIE + ESIPT” characteristics for specific detection of lysosomal esterase

Supplementary files

Article information

Article type
Paper
Submitted
01 Mar 2014
Accepted
07 Apr 2014
First published
07 Apr 2014

J. Mater. Chem. B, 2014,2, 3438-3442

Author version available

A fluorescent light-up probe with “AIE + ESIPT” characteristics for specific detection of lysosomal esterase

M. Gao, Q. Hu, G. Feng, B. Z. Tang and B. Liu, J. Mater. Chem. B, 2014, 2, 3438 DOI: 10.1039/C4TB00345D

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