Issue 8, 2015

Hemin-functionalized WS2 nanosheets as highly active peroxidase mimetics for label-free colorimetric detection of H2O2 and glucose

Abstract

Hemin-functionalized WS2 nanosheets (hemin/WS2-NSs) were first obtained by hemin assembled on the surface of few-layered WS2 nanosheets (WS2-NSs) via van der Waals interactions. Significantly, this new material possessed the advantages of both hemin and WS2 nanosheets and exhibited some unique properties. Firstly, hemin/WS2-NSs had intrinsic peroxidase-like activity, which could effectively catalyze oxidation of the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2 to produce a typical blue colored reaction. Secondly, the activity of hemin/WS2-NSs was much higher than the activity of hemin or WS2-NSs alone. The catalytic activity followed the typical Michaelis–Menten kinetics and was dependent on the temperature, pH, H2O2 concentration, as well as reaction time. Based on this finding, a new highly sensitive and selective colorimetric method for H2O2 and glucose detection was developed. This method was simple and inexpensive for glucose detection using glucose oxidase (GOx) and hemin/WS2-NSs with a linear range of 0.5 × 10−5 to 2.0 × 10−4 mol L−1 with a detection limit of 1.5 × 10−6 mol L−1. The good catalytic activity and low-cost make the hemin/WS2-NSs a useful biocatalyst for a wide range of potential applications in environmental chemistry, biotechnology and clinical diagnostics.

Graphical abstract: Hemin-functionalized WS2 nanosheets as highly active peroxidase mimetics for label-free colorimetric detection of H2O2 and glucose

Supplementary files

Article information

Article type
Paper
Submitted
07 Jan 2015
Accepted
19 Feb 2015
First published
19 Feb 2015

Analyst, 2015,140, 2857-2863

Author version available

Hemin-functionalized WS2 nanosheets as highly active peroxidase mimetics for label-free colorimetric detection of H2O2 and glucose

Q. Chen, J. Chen, C. Gao, M. Zhang, J. Chen and H. Qiu, Analyst, 2015, 140, 2857 DOI: 10.1039/C5AN00031A

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