Issue 14, 2015

Nanopore analysis of amyloid fibrils formed by lysozyme aggregation

Abstract

The measurement of single particle size distributions of amyloid fibrils is crucial for determining mechanisms of growth and toxicity. Nanopore sensing is an attractive solution for this problem since it gives information on aggregates’ shapes with relatively high throughput for a single particle technology. In this paper we study the translocation of lysozyme fibrils through quartz glass nanopores. We demonstrate that, under appropriate salt and pH conditions, lysozyme fibrils translocate through bare quartz nanopores without causing significant clogging. This enables us to measure statistics on tens of thousands of translocations of lysozyme fibrils with the same nanopore and track their development over a time course of aggregation spanning 24 h. Analysis of our events shows that the statistics are consistent with a simple bulk conductivity model for the passage of rods with a fixed cross sectional area through a conical glass nanopore.

Graphical abstract: Nanopore analysis of amyloid fibrils formed by lysozyme aggregation

Supplementary files

Article information

Article type
Paper
Submitted
17 Mar 2015
Accepted
12 May 2015
First published
12 May 2015
This article is Open Access
Creative Commons BY license

Analyst, 2015,140, 4882-4886

Author version available

Nanopore analysis of amyloid fibrils formed by lysozyme aggregation

N. Martyushenko, N. A. W. Bell, R. D. Lamboll and U. F. Keyser, Analyst, 2015, 140, 4882 DOI: 10.1039/C5AN00530B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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