Issue 15, 2015

Confined chemiluminescence detection of nanomolar levels of H2O2 in a paper–plastic disposable microfluidic device using a smartphone

Abstract

We report the design and characterization of a disposable light shielded paper–plastic microfluidic device that can detect nanomolar levels of H2O2 using a smartphone camera and a light sealed accessory. Chemiluminescence reaction of H2O2 with bis(2,4,6-trichlorophenyl)oxalate in the presence of rubrene and imidazole is confined to a paper reaction site where the individual reagents are delivered via plastic microfluidic channels. The net photon emission from the chemiluminescence reactions, detected by using a smartphone, is correlated with H2O2 concentrations. With a total reagent volume of 25 μL, the sensor system was able to detect H2O2 concentrations as low as 250 nM. The smartphone based chemiluminescence sensing system has great potential as a point of care diagnostic tool for monitoring nanomolar levels of H2O2 in biological samples.

Graphical abstract: Confined chemiluminescence detection of nanomolar levels of H2O2 in a paper–plastic disposable microfluidic device using a smartphone

Supplementary files

Article information

Article type
Communication
Submitted
14 Apr 2015
Accepted
27 May 2015
First published
27 May 2015

Analyst, 2015,140, 5006-5011

Author version available

Confined chemiluminescence detection of nanomolar levels of H2O2 in a paper–plastic disposable microfluidic device using a smartphone

E. Lebiga, R. Edwin Fernandez and A. Beskok, Analyst, 2015, 140, 5006 DOI: 10.1039/C5AN00720H

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