Application of inductively coupled plasma mass spectrometry in the study of apoptosis: determination of caspase-3 using a gold nanoparticle tag

Abstract

Apoptosis is a main type of cell death in which caspase-3 plays a key role. In this work, a simple, fast and sensitive immunoassay for caspase-3 is established by using inductively coupled plasma mass spectrometry (ICP-MS) detection and signal enhancement gold nanoparticle (Au-NP) labelling of the secondary antibody (IgG). After the immunoreaction of caspase-3, primary antibody and Au-NP labeled IgG, the concentration of caspase-3 was determined by ICP-MS analysis of the Au-NPs released from the immunocomplex. Under the optimized conditions, a limit of detection (LOD, 3σ) of 0.42 ng mL⁻¹ (0.31 nM) was obtained for caspase-3, with a dynamic range of 1–200 ng mL⁻¹ and a relative standard deviation of 4.1% (c = 5 ng mL⁻¹, n = 11). The proposed method has good specificity towards caspase-3. To demonstrate the application potential of the proposed method, the cell lysates of Hg²⁺-induced HepG2 cells were analyzed, and it was found that the concentration of caspase-3 increased when increasing the incubation concentration of Hg²⁺. This method is easy, sensitive and selective, and has excellent capability of tolerating a complex biological matrix, indicating the potential of the ICP-MS-based assay in the study of apoptosis.