Issue 47, 2015
From the journal:

Chemical Communications

Photo-triggered fluorescent labelling of recombinant proteins in live cells†

Deokho Jung,a   Kohei Sato,b   Kyoungmi Min,a   Akira Shigenaga,b   Juyeon Jung,c   Akira Otaka*b  and  Youngeun Kwon*a  

* Corresponding authors

a Department of Biomedical Engineering, Dongguk University-Seoul, Pildong 3-ga, Seoul, Korea
E-mail: ykwon@dongguk.edu
Fax: +82-2-2260-8769
Tel: +82-2-2260-3320

b Institute of Health Biosciences and Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78-1 Shomachi, Tokushima, Japan
E-mail: aotaka@tokushima-u.ac.jp
Fax: +81-88-633-7283
Tel: +81-88-633-9505

c BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, Korea

Abstract

A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.

Graphical abstract: Photo-triggered fluorescent labelling of recombinant proteins in live cells

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Article information

DOI
https://doi.org/10.1039/C5CC01067E
Article type
Communication
Submitted
05 Feb 2015
Accepted
05 May 2015
First published
15 May 2015

Chem. Commun., 2015,51, 9670-9673

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Photo-triggered fluorescent labelling of recombinant proteins in live cells

D. Jung, K. Sato, K. Min, A. Shigenaga, J. Jung, A. Otaka and Y. Kwon, Chem. Commun., 2015, 51, 9670 DOI: 10.1039/C5CC01067E

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