Issue 50, 2015
From the journal:

Chemical Communications

Design of enzyme-interfaced DNA logic operations (AND, OR and INHIBIT) with an assaying application for single-base mismatch†

Long Ma*abc  and  Aipo Diaoab  

* Corresponding authors

a Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
E-mail: malong@tust.edu.cn
Fax: +86 22 60602298
Tel: +86 22 60602948

b Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
Tel: +86 33 4467254

c Biomolecular Sciences Research Complex, EaStCHEM School of Chemistry, University of St Andrews, UK
E-mail: lm228@st.andrews.ac.uk

Abstract

We devised AND, OR and INHIBIT logic gates. They were based on the enzyme-induced DNA base flipping mechanism, which caused significant local conformation changes in DNA. This can be monitored via photonic signals as outputs. We also provided a prototype of a built-in biosensor capable of distinguishing single-base mismatches in a DNA duplex.

Graphical abstract: Design of enzyme-interfaced DNA logic operations (AND, OR and INHIBIT) with an assaying application for single-base mismatch

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Article information

DOI
https://doi.org/10.1039/C5CC02835C
Article type
Communication
Submitted
06 Apr 2015
Accepted
08 May 2015
First published
29 May 2015

Chem. Commun., 2015,51, 10233-10235

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Design of enzyme-interfaced DNA logic operations (AND, OR and INHIBIT) with an assaying application for single-base mismatch

L. Ma and A. Diao, Chem. Commun., 2015, 51, 10233 DOI: 10.1039/C5CC02835C

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