A highly selective and fast-response fluorescent probe for visualization of enzymatic H2S production in vitro and in living cells

Lv Wei; Zhentao Zhu; Yanyan Li; Long Yi; Zhen Xi

doi:10.1039/C5CC03707G

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A highly selective and fast-response fluorescent probe for visualization of enzymatic H₂S production in vitro and in living cells†

Lv Wei,‡^ac Zhentao Zhu,‡^b Yanyan Li,^ac Long Yi*^bc and Zhen Xi*^ac

Author affiliations

* Corresponding authors

^a State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University, Tianjin, China
E-mail: zhenxi@nankai.edu.cn
Tel: +86 22 23504782

^b State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China
E-mail: yilong@mail.buct.edu.cn

^c Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China

Abstract

An o-fluorinated-azido-capped rhodamine probe can react with H₂S efficiently and selectively to give large off–on fluorescence enhancement. The probe was used to develop an assay for cystathionine β-synthase acitivity and for in situ visualization of endogenously produced H₂S in living cells.

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Article information

DOI: https://doi.org/10.1039/C5CC03707G
Article type: Communication
Submitted: 04 May 2015
Accepted: 19 May 2015
First published: 19 May 2015

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Chem. Commun., 2015,51, 10463-10466

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A highly selective and fast-response fluorescent probe for visualization of enzymatic H₂S production in vitro and in living cells

L. Wei, Z. Zhu, Y. Li, L. Yi and Z. Xi, Chem. Commun., 2015, 51, 10463 DOI: 10.1039/C5CC03707G

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Chemical Communications