Issue 70, 2015
From the journal:

Chemical Communications

Label-free molecular beacon-based quadratic isothermal exponential amplification: a simple and sensitive one-pot method to detect DNA methyltransferase activity†

Qingwang Xue,ab   Lei Wang*a  and  Wei Jiang*c  

* Corresponding authors

a School of Pharmacy, Shandong University, Jinan 250012, P. R. China
E-mail: wangl-sdu@sdu.edu.cn
Fax: +86-531-88564464
Tel: +86-531-88363888

b Department of Chemistry, Liaocheng University, Liaocheng, Shandong, China

c School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China
E-mail: wjiang@sdu.edu.cn

Abstract

We developed a one-pot label-free molecular beacon-mediated quadratic isothermal exponential amplification strategy (LFMB-QIEA) for simple, rapid and sensitive DNA methyltransferase (MTase) activity detection.

Graphical abstract: Label-free molecular beacon-based quadratic isothermal exponential amplification: a simple and sensitive one-pot method to detect DNA methyltransferase activity

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Article information

DOI
https://doi.org/10.1039/C5CC05410A
Article type
Communication
Submitted
01 Jul 2015
Accepted
20 Jul 2015
First published
20 Jul 2015

Chem. Commun., 2015,51, 13538-13541

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Label-free molecular beacon-based quadratic isothermal exponential amplification: a simple and sensitive one-pot method to detect DNA methyltransferase activity

Q. Xue, L. Wang and W. Jiang, Chem. Commun., 2015, 51, 13538 DOI: 10.1039/C5CC05410A

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