A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase

D. Baud; N. Ladkau; T. S. Moody; J. M. Ward; H. C. Hailes

doi:10.1039/C5CC06817G

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A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase†

D. Baud,^a N. Ladkau,^a T. S. Moody,^b J. M. Ward^c and H. C. Hailes*^a

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* Corresponding authors

^a Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK
E-mail: h.c.hailes@ucl.ac.uk
Tel: +44 (0)20 7679 7463

^b Almac, Department of Biocatalysis and Isotope Chemistry, 20 Seagoe Industrial Estate, Craivagon, N. Ireland, UK

^c Department of Biochemical Engineering, University College London, Gordon Street, London WC1H 0AH, UK

Abstract

A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.

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Article information

DOI: https://doi.org/10.1039/C5CC06817G
Article type: Communication
Submitted: 13 Aug 2015
Accepted: 23 Sep 2015
First published: 24 Sep 2015
This article is Open Access

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Chem. Commun., 2015,51, 17225-17228

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A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase

D. Baud, N. Ladkau, T. S. Moody, J. M. Ward and H. C. Hailes, Chem. Commun., 2015, 51, 17225 DOI: 10.1039/C5CC06817G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Chemical Communications

A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase†

Abstract

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A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase

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