Issue 88, 2015

Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-l-phenylalanine

Abstract

Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-L-phenylalanine via Cu(I)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+–Gd3+ distance measurements by double electron–electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.

Graphical abstract: Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-l-phenylalanine

Supplementary files

Article information

Article type
Communication
Submitted
25 Aug 2015
Accepted
15 Sep 2015
First published
15 Sep 2015

Chem. Commun., 2015,51, 15898-15901

Author version available

Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine

E. H. Abdelkader, A. Feintuch, X. Yao, L. A. Adams, L. Aurelio, B. Graham, D. Goldfarb and G. Otting, Chem. Commun., 2015, 51, 15898 DOI: 10.1039/C5CC07121F

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