Issue 6, 2016
From the journal:

Chemical Communications

Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection†

Fei Ma,‡ab   Meng Liu,‡a   Zi-yue Wang‡a  and  Chun-yang Zhang*ab  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn
Fax: +86 0531-82615258
Tel: +86 0531-86186033

b Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

Abstract

We develop a sensitive and selective method for the multiplex detection of histone-modifying enzymes (HMEs) through the integration of antibody-based fluorescence labeling with total internal reflection fluorescence (TIRF)-based single-molecule detection. This method exhibits excellent specificity and high sensitivity with a detection limit of 21 pM for histone acetyltransferase GcN5 and 12 pM for histone methyltransferase G9a, and it can be applied for the screening of HME inhibitors as well.

Graphical abstract: Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC08797J
Article type
Communication
Submitted
23 Oct 2015
Accepted
17 Nov 2015
First published
17 Nov 2015

Chem. Commun., 2016,52, 1218-1221

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Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection

F. Ma, M. Liu, Z. Wang and C. Zhang, Chem. Commun., 2016, 52, 1218 DOI: 10.1039/C5CC08797J

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