Issue 11, 2015

Dual mode quantitative imaging of microscopic viscosity using a conjugated porphyrin dimer

Abstract

Microviscosity is of paramount importance in materials and bio-sciences. Fluorescence imaging using molecular rotors has emerged as a versatile tool to measure microviscosity, either using a fluorescence lifetime or a ratiometric signal of the rotor; however, only a limited number of blue-to-green-emitting fluorophores with both the lifetime and the ratiometric signal sensitivity to viscosity have been reported to date. Here we report a deep red emitting dual viscosity sensor, which allows both the ratiometric and the lifetime imaging of viscosity. We study viscosity in a range of lipid-based systems and conclude that in complex dynamic systems dual detection is preferable in order to independently verify the results of the measurements as well as perform rapid detection of changing viscosity.

Graphical abstract: Dual mode quantitative imaging of microscopic viscosity using a conjugated porphyrin dimer

Supplementary files

Article information

Article type
Paper
Submitted
23 Jan 2015
Accepted
16 Feb 2015
First published
17 Feb 2015

Phys. Chem. Chem. Phys., 2015,17, 7548-7554

Author version available

Dual mode quantitative imaging of microscopic viscosity using a conjugated porphyrin dimer

A. Vyšniauskas, M. Balaz, H. L. Anderson and M. K. Kuimova, Phys. Chem. Chem. Phys., 2015, 17, 7548 DOI: 10.1039/C5CP00439J

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