Issue 7, 2015

Interaction of a novel platinum drug with bovine serum albumin: FTIR and UV-Vis spectroscopy analysis

Abstract

Platinum complexes have proven to be very effective in cancer treatment. However, severe side effects of these drugs have lead scientists to pursue new platinum complex derivatives. A novel blue platinum compound, called Platinum-Blue (Pt-Blue), is one of the promising candidate platinum compounds to be used for tumor treatment. In this study, the interaction of Pt-Blue with bovine serum albumin (BSA) has been investigated using UV-Vis and FTIR spectroscopy. One of the findings is that the drug-protein interaction type depends on the drug concentration. Though Pt-Blue is attached to the surface of BSA at high concentrations, it interacts with a hydrophobic region of the protein at low concentrations with a binding constant of 1.93 × 105 M−1. Spectroscopic results indicate the hydrophobic docking position to be around Trp 213 in domain II, which is surrounded by a number of Asp and Glu. During this interaction, helices such as helix-10, helix-18, helix-19 and helix-24 change orientation and/or partially unfold to make room for the compound. Binding constants at high and low concentrations of Pt-Blue are determined using UV-Vis spectroscopy, which are found to be comparable to cisplatin. FTIR spectroscopy also reveals that the interaction between Pt-Blue and BSA is noncovalent, which makes the candidate drug favorable because it is available for DNA binding while being carried by albumin.

Graphical abstract: Interaction of a novel platinum drug with bovine serum albumin: FTIR and UV-Vis spectroscopy analysis

Supplementary files

Article information

Article type
Paper
Submitted
29 Mar 2015
Accepted
12 May 2015
First published
13 May 2015

New J. Chem., 2015,39, 5676-5685

Author version available

Interaction of a novel platinum drug with bovine serum albumin: FTIR and UV-Vis spectroscopy analysis

F. Korkmaz, D. A. Erdogan and Ş. Özalp-Yaman, New J. Chem., 2015, 39, 5676 DOI: 10.1039/C5NJ00785B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements