Issue 7, 2016

Computational design of peptide-Au cluster probe for sensitive detection of αIIbβ3 integrin

Abstract

We have designed a novel peptide-Au cluster probe to specifically bind to αIIbβ3 integrin. As indicated by molecular dynamics (MD) simulations, the binding mode of the native ligand of αIIbβ3 integrin, γC peptide, can be realized by the designed probe. More importantly, the peptide-Au probe can provide multiple coating peptides to form additional salt bridges with protein, and the binding stability of the probe is comparable to the native ligand. The designed probe was then successfully synthesized. The specific binding in a cellular environment was validated by colocalization analysis of confocal microscopy. In addition, the binding affinity was confirmed by atomic force microscopy (AFM) based single molecule force spectroscopy. Our results suggest the combination of computational design and experimental verification can be a useful strategy for the development of nanoprobes.

Graphical abstract: Computational design of peptide-Au cluster probe for sensitive detection of αIIbβ3 integrin

Supplementary files

Article information

Article type
Paper
Submitted
24 Dec 2015
Accepted
21 Jan 2016
First published
25 Jan 2016

Nanoscale, 2016,8, 4203-4208

Computational design of peptide-Au cluster probe for sensitive detection of αIIbβ3 integrin

L. Zhao, J. Zhai, X. Zhang, X. Gao, X. Fang and J. Li, Nanoscale, 2016, 8, 4203 DOI: 10.1039/C5NR09175F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements