Issue 29, 2015

A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine

Abstract

Herein we report the use of bromomaleimides for the construction of stable albumin conjugates via conjugation to its native, single accessible, cysteine followed by hydrolysis. Advantages over the classical maleimide approach are highlighted in terms of quantitative hydrolysis and absence of undesirable retro-Michael deconjugation.

Graphical abstract: A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine

Supplementary files

Article information

Article type
Paper
Submitted
12 Jun 2015
Accepted
17 Jun 2015
First published
17 Jun 2015
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2015,13, 7946-7949

A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine

M. E. B. Smith, M. B. Caspersen, E. Robinson, M. Morais, A. Maruani, J. P. M. Nunes, K. Nicholls, M. J. Saxton, S. Caddick, J. R. Baker and V. Chudasama, Org. Biomol. Chem., 2015, 13, 7946 DOI: 10.1039/C5OB01205H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements