Issue 54, 2015
From the journal:

RSC Advances

Label-free and amplified colorimetric assay of ribonuclease H activity and inhibition based on a novel enzyme-responsive DNAzyme cascade†

Shulan Zeng,a   Huakui Huang,ab   Yong Huang,*ab   Xiaoqian Liu,b   Jian Qin,b   Shulin Zhao,*ab   Zhen-Feng Chena  and  Hong Liang*ab  

* Corresponding authors

a Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, China
E-mail: huangyong_2009@163.com, zhaoshulin001@163.com, hliang@gxnu.edu.cn

b College of Chemistry and Pharmacy, Guangxi Normal University, Guilin, China

Abstract

A simple, label-free and amplified colorimetric assay strategy based on a novel enzyme-responsive DNAzyme cascade has been developed for assay of ribonuclease H activity and inhibition. This novel strategy improved the detection sensitivity by two orders of magnitude over the previously reported methods.

Graphical abstract: Label-free and amplified colorimetric assay of ribonuclease H activity and inhibition based on a novel enzyme-responsive DNAzyme cascade

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Article information

DOI
https://doi.org/10.1039/C5RA05712D
Article type
Communication
Submitted
31 Mar 2015
Accepted
05 May 2015
First published
05 May 2015

RSC Adv., 2015,5, 43105-43109

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Label-free and amplified colorimetric assay of ribonuclease H activity and inhibition based on a novel enzyme-responsive DNAzyme cascade

S. Zeng, H. Huang, Y. Huang, X. Liu, J. Qin, S. Zhao, Z. Chen and H. Liang, RSC Adv., 2015, 5, 43105 DOI: 10.1039/C5RA05712D

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