Issue 95, 2015
From the journal:

RSC Advances

Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein†‡

Samuel C. Reddington,§a   Sarunas Driezis,§a   Andrew M. Hartley,§a   Peter D. Watson,a   Pierre J. Rizkallahb  and  D. Dafydd Jones*a  

* Corresponding authors

a School of Biosciences, Cardiff University, Cardiff, UK
E-mail: jonesdd@cardiff.ac.uk
Tel: +44 (0)2920 20874290

b School of Medicine, Cardiff University, Cardiff, UK

Abstract

The photochemical properties of phenyl azide have been exploited to modulate the function of a red autofluorescent protein, mCherry. Using genetic code reprogramming, phenyl azide chemistry has been introduced at functionally strategic positions in mCherry leading to deactivation, activation or enhancement upon UV irradiation.

Graphical abstract: Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein

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Article information

DOI
https://doi.org/10.1039/C5RA13552D
Article type
Communication
Submitted
10 Jul 2015
Accepted
07 Sep 2015
First published
08 Sep 2015
This article is Open Access
Creative Commons BY license

RSC Adv., 2015,5, 77734-77738

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Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein

S. C. Reddington, S. Driezis, A. M. Hartley, P. D. Watson, P. J. Rizkallah and D. D. Jones, RSC Adv., 2015, 5, 77734 DOI: 10.1039/C5RA13552D

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