Issue 125, 2015

A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin

Abstract

It is well known that Cu(II) can coordinate with L-alanine (Cu–Ala), which can be destroyed through the addition of glutamic-pyruvic transaminase (GPT) since GPT can effectively catalyze the conversion of L-alanine into keto-acetic acid. As a result, the free Cu(II) ion can combine with bovine serum albumin (BSA) and in turn quench the fluorescence of BSA. In this context, a simple and sensitive GPT activity detection via fluorescence quenching method has been developed. The fluorescence intensity of the system shows a linear relationship with the GPT concentration in the range of 5 and 400 U L−1 with a detection limit down to 3 U L−1 (S/N = 3). Avoiding any labels or complicated operations, this cost-effective and convenient method holds the potential for the rapid diagnosis of GPT-related diseases.

Graphical abstract: A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin

Article information

Article type
Paper
Submitted
16 Nov 2015
Accepted
25 Nov 2015
First published
26 Nov 2015

RSC Adv., 2015,5, 103557-103562

A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin

M. Chen, L. Rong and X. Chen, RSC Adv., 2015, 5, 103557 DOI: 10.1039/C5RA24162F

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