Issue 3, 2016
From the journal:

Chemical Science

Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions†

Shan Tang,‡a   Zhengpeng Wan,‡b   Yiren Gao,‡b   Ji-Shen Zheng,c   Jing Wang,b   Yan-Yan Si,a   Xin Chen,d   Hai Qi,d   Lei Liu*a  and  Wanli Liu*b  

* Corresponding authors

a Tsinghua-Peking Center for Life Sciences, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China
E-mail: lliu@mail.tsinghua.edu.cn

b MOE Key Laboratory of Protein Science, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Life Sciences, Tsinghua University, Beijing, China
E-mail: liulab@mail.tsinghua.edu.cn

c High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, China

d Laboratory of Dynamic Immunobiology, School of Medicine, Tsinghua University, Beijing, China

Abstract

We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen–antibody interaction mediated responses in B cells. This strategy facilitated fine tuning of the caged protein antigen to optimize its bioactivity and photochemical properties. One optimal molecule, HEL-K96NPE, was totally inert to hen egg lysozyme (HEL)-specific B cells and could only restore its antigenicity upon photoactivation. Combined with real time live cell imaging, the utility of HEL-K96NPE was demonstrated as a proof of concept to quantify B cell synapse formation and calcium influx responses at the single cell level.

Graphical abstract: Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions

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Article information

DOI
https://doi.org/10.1039/C5SC03404C
Article type
Edge Article
Submitted
10 Sep 2015
Accepted
19 Nov 2015
First published
11 Dec 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2016,7, 1891-1895

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Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions

S. Tang, Z. Wan, Y. Gao, J. Zheng, J. Wang, Y. Si, X. Chen, H. Qi, L. Liu and W. Liu, Chem. Sci., 2016, 7, 1891 DOI: 10.1039/C5SC03404C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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