Issue 6, 2016

A carboxylesterase-selective ratiometric fluorescent two-photon probe and its application to hepatocytes and liver tissues

Abstract

Carboxylesterases (CEs) are widely distributed enzymes in the human body that catalyze hydrolysis of various endogenous and exogenous substrates. They are directly linked to hepatic drug metabolisms and steatosis, and their regulations are important issues in pharmacological and clinical applications. In this work, we have developed an emission ratiometric two-photon probe (SE1) for quantitatively detecting CE in situ. This probe is based on a translation of intramolecular charge transfer character upon reaction with CE. It shows a sensitive blue-to-yellow emission change in response to human CE activity, easy loading into cells, insensitivity to pH and other metabolites including ROS and RNS, high photostability, and low cytotoxicity. Using live hepatocytes and liver tissues, we found that ratiometric two-photon microscopic imaging with SE1 is an effective tool for monitoring CE activities at the subcellular level in live tissues. This probe will find useful applications in biomedical research, including studies of hepatic steatosis and drug developments.

Graphical abstract: A carboxylesterase-selective ratiometric fluorescent two-photon probe and its application to hepatocytes and liver tissues

Supplementary files

Article information

Article type
Edge Article
Submitted
30 Dec 2015
Accepted
23 Feb 2016
First published
23 Feb 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2016,7, 3703-3709

A carboxylesterase-selective ratiometric fluorescent two-photon probe and its application to hepatocytes and liver tissues

S. J. Park, H. W. Lee, H. Kim, C. Kang and H. M. Kim, Chem. Sci., 2016, 7, 3703 DOI: 10.1039/C5SC05001D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements