Issue 12, 2015

A size dependent evaluation of the cytotoxicity and uptake of nanographene oxide

Abstract

Graphene oxide (GO) has attracted great interest due to its extraordinary potential for biomedical application. Although it is clear that the naturally occurring morphology of biological structures is crucial to their precise interactions and correct functioning, the geometrical aspects of nanoparticles are often ignored in the design of nanoparticles for biological applications. A few in vitro and in vivo studies have evaluated the cytotoxicity and biodistribution of GO, however very little is known about the influence of flake size and cytotoxicity. Herein, we aim at presenting an initial cytotoxicity evaluation of different nano-sized GO flakes for two different cell lines (HeLa (Kyoto) and macrophage (J7742)) when they are exposed to samples containing different sized nanographene oxide (NGO) flakes (mean diameter of 89 and 277 nm). The obtained data suggests that the larger NGO flakes reduce cell viability as compared to smaller flakes. In addition, the viability reduction correlates with the time and the concentration of the NGO nanoparticles to which the cells are exposed. Uptake studies were also conducted and the data suggests that both cell lines internalize the GO nanoparticles during the incubation periods studied.

Graphical abstract: A size dependent evaluation of the cytotoxicity and uptake of nanographene oxide

Supplementary files

Article information

Article type
Paper
Submitted
25 Jan 2015
Accepted
10 Feb 2015
First published
12 Feb 2015
This article is Open Access
Creative Commons BY-NC license

J. Mater. Chem. B, 2015,3, 2522-2529

Author version available

A size dependent evaluation of the cytotoxicity and uptake of nanographene oxide

R. G. Mendes, B. Koch, A. Bachmatiuk, X. Ma, S. Sanchez, C. Damm, O. G. Schmidt, T. Gemming, J. Eckert and M. H. Rümmeli, J. Mater. Chem. B, 2015, 3, 2522 DOI: 10.1039/C5TB00180C

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements