Issue 26, 2015

An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

Abstract

An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.

Graphical abstract: An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

Supplementary files

Article information

Article type
Communication
Submitted
25 Mar 2015
Accepted
05 Jun 2015
First published
08 Jun 2015

J. Mater. Chem. B, 2015,3, 5166-5171

Author version available

An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

C. C. Mayorga-Martinez, A. Chamorro-García, L. Serrano, L. Rivas, D. Quesada-Gonzalez, L. Altet, O. Francino, A. Sánchez and A. Merkoçi, J. Mater. Chem. B, 2015, 3, 5166 DOI: 10.1039/C5TB00545K

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