Issue 47, 2015

A fluorescent light-up probe based on AIE and ESIPT processes for β-galactosidase activity detection and visualization in living cells

Abstract

A novel fluorescent probe SA-βGal is reported here with light-up response to β-galactosidase. SA-βGal possesses the β-galactopyranoside group to react with β-galactosidase and releases the fluorescent salicylaldehyde azine with both aggregation induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) characteristics. The linear fluorescent response enables the in vitro quantification of β-galactosidase activity in a range of 0–0.1 U mL−1 with a detection limit of 0.014 U mL−1. The probe exhibits significant advantages, such as no self-quenching at high concentrations, a large Stokes shift (190 nm) and high specificity to β-galactosidase with an excellent light-up ratio of 820 fold. Moreover, thanks to its good retention in living cells, the application of SA-βGal for the imaging of cellular β-galactosidase was also achieved with high contrast.

Graphical abstract: A fluorescent light-up probe based on AIE and ESIPT processes for β-galactosidase activity detection and visualization in living cells

Supplementary files

Article information

Article type
Paper
Submitted
17 Sep 2015
Accepted
30 Oct 2015
First published
02 Nov 2015

J. Mater. Chem. B, 2015,3, 9168-9172

Author version available

A fluorescent light-up probe based on AIE and ESIPT processes for β-galactosidase activity detection and visualization in living cells

L. Peng, M. Gao, X. Cai, R. Zhang, K. Li, G. Feng, A. Tong and B. Liu, J. Mater. Chem. B, 2015, 3, 9168 DOI: 10.1039/C5TB01938A

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