Issue 34, 2016

Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

Abstract

Nonspecific protein adsorption, referred to as fouling, is a major clinical problem accompanying any material coming into direct contact with biological fluids, especially blood/plasma. Not only is protein fouling detrimental per se but it also promotes platelet adhesion, the initiation of blood coagulation, and/or complement activation. In spite of efforts to develop novel surfaces which suppress protein fouling, still only little is known about the mechanisms behind protein fouling from blood and other complex biological media. In order to unravel the mechanisms, precise identification of the proteins that foul the surface is necessary. This is particularly challenging for those surfaces with reduced protein fouling, such as polymer brushes. This report introduces a route to analyze the proteins by mass spectrometry using an optimized protocol. We demonstrate proteomics-compatible buffers/solutions suitable for desorption of adsorbed proteins on low fouling polymer brushes. In this way, not only mass spectrometry, but also a plethora of other studies based on state-of-the-art proteomics can be accessed.

Graphical abstract: Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

Article information

Article type
Paper
Submitted
28 Jun 2016
Accepted
24 Jul 2016
First published
28 Jul 2016

Anal. Methods, 2016,8, 6415-6419

Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

T. Riedel, P. Májek, Z. Riedelová-Reicheltová, M. Vorobii, M. Houska and C. Rodriguez-Emmenegger, Anal. Methods, 2016, 8, 6415 DOI: 10.1039/C6AY01833E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements