Issue 31, 2016

A label-free electrochemical immunosensor for beta-amyloid detection

Abstract

A label-free detection of beta-amyloid (βA) protein using an electrochemical immunosensor fabricated via immobilizing specific anti-beta-amyloid antibodies (An-βA-Abs) onto an interdigitated electrode of gold (IDE-Au) modified using a self-assembled monolayer (SAM) of dithiobis(succinimidyl propionate) [DTSP] is presented here. The βA has been investigated as a potential biomarker for monitoring Alzheimer's disease (AD), permanent irreversible and progressive brain damage. Thus βA detection at the pM level is of high significance for AD diagnostics. The IDE-Au modification and covalent immobilization of An-βA-Abs onto electrodes were characterized by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) as a function of electrical response variation in each step involved in sensor fabrication. The EIS studies confirmed that the developed βA immunosensor is selective and exhibits a detection limit of 10 pM, its detection range varies from 10 pM to 100 nM, and it has a high sensitivity of 11 kΩ M−1 with a regression coefficient of 0.99. Thus, the developed sensitive and selective immunosensor with the features of the IDE-Au can be integrated with a miniaturized potentiostat (M-P) to develop a sensing system to detect βA for point-of-care (POC) applications for the assessment and management of AD. The bio-informatics gathered from such a system could be useful to make timely therapeutic decisions.

Graphical abstract: A label-free electrochemical immunosensor for beta-amyloid detection

Article information

Article type
Paper
Submitted
05 Jul 2016
Accepted
09 Jul 2016
First published
11 Jul 2016

Anal. Methods, 2016,8, 6115-6120

A label-free electrochemical immunosensor for beta-amyloid detection

A. Kaushik, P. Shah, P. K. Vabbina, R. D. Jayant, S. Tiwari, A. Vashist, A. Yndart and M. Nair, Anal. Methods, 2016, 8, 6115 DOI: 10.1039/C6AY01910B

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