Exploring substrate scope and stereoselectivity of P450 peroxygenase OleTJE in olefin-forming oxidative decarboxylation

Abstract

As recently reported, the olefin-forming oxidative decarboxylation of straight-chain C₄–C₂₂ carboxylic acids catalyzed by P450 peroxygenase OleT_JE constitutes a mild alkene synthesis. The present study shows that structurally different carboxylic acids are also accepted, including those that generate di-substituted olefins. Exploratory mutational experiments lead to increased trans-selectivity.